Thymoquinone Ameliorates Acute Kidney Injury Induced by Renal Ischemia-Reperfusion

Renal ischemia-reperfusion injury (IRI)is an unavoidable consequence in renal transplantation and multiple clinical settings. A debate has been raised about the particular role of hypoxia-inducible factor (HF-1α) pathogenesis cortex ultrastructural alterations. Also, we investigated antioxidant/anti-inflammatory effect thymoquinone its modulatory on HIF-1α protection against IRI.Adult male Wister albino rats were assigned into 3 groups (n=16); 1) Sham-operated, 2) IRI model 3) pre-treated with 10 mg.kg-1.day-1 (TQ-IRI) for days at reperfusion onset. Following operation, 8 from each group euthanized after hours remaining 24 hours. was assessed by increased blood urea nitrogen, creatinine level, EGTI histological scoreat both 24h. upregulated (p<0.01) correlated tissue reactive oxygen species (ROS) production total oxidant capacity (TAC) consumption. Elevated inflammatory markers (NFkB, MCP-1 VCAM-1) associated IRI.Thymoquinone treatment inhibited accumulation (p<0.01), reduced oxidation/inflammation process markedly diminished injury.